Enzymes and Inhibitors
MOLDPRO INC Sample Holder for RDE-OES, ~2 mL, 18 x 9.5 mm, Black, 1000/pkg, Flat bottom, PP Homopolymer
MP-116 Sample holder is made to fit SpectrOil M Series Rotating Disc Electrode Optical Emission Spectroscope (RDEOES). Comparable to Spectro Scientific M90200 & P-10524, SPC Science 070-010-101 and NSN 6640-01-445-1330. Made with Polypropylene homopolymer and packaged 1000 cups/pack.• Used for sample presentation to SpectrOil M/N-W, M/C-W, M/F-W, M/R and 100 series instruments• Can be used manually or with Double Disc Rapid Robot (D2R2)• Used for ASTM D6595 and ASTM D6728 test methods
Vector Laboratories VECTOR Blue Alkaline Phosphatase (AP) Substrate Kit 200 ml
VECTOR Blue Alkaline Phosphatase (AP) Substrate Kit produces a blue, heat-stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. Sections stained with the VECTOR Blue substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount) must be used because the reaction product is soluble in xylene. VECTOR Blue can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. The stock solutions in this kit are provided in convenient dropper bottles, with sufficient volume for 200 ml working solution.
New England Biolabs, Inc. BclI-HF – 3000 units
A high fidelity restriction endonuclease that recognizes the sequence T^GATCA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. TspMI – 200 units
A restriction endonuclease that recognizes the sequence C^CCGG_G.
Teknova qPCR Proteinase K Buffer (1.11M NaCl, 1.11% Sarkosyl). 100mL, Sterile.
1.11M Sodium Chloride,1.11% Sarkosyl
STEMCELL Technologies ArciTect Cas9-eGFP Nuclease , Size: 100 ug
Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease (3 μg/ul) for the generation of double-strand breaks in CRISPR-Cas9 genome editing
New England Biolabs, Inc. EnGen Spy Cas9 HF1 - 500 pmol
EnGen Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from Streptococcus pyogenes with reduced non-specific DNA cleavage. Spy Cas9 is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The single guide RNA (sgRNA) targets Cas9 to the region immediately upstream of a 5'-NGG-3' protospacer adjacent motif (PAM) producing a double stranded break 3 bases upstream of the PAM. EnGen Spy Cas9 HF1 contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C-termini of the protein.
New England Biolabs, Inc. NEBExpress T4 Lysozyme
NEBExpress T4 Lysozyme is a recombinant murein hydrolase that breaks down the bacterial cell wall by hydrolyzing the beta 1-4 linkage between N-acetylmuramic acid and N-acetylglucosamine in the peptidoglycan of prokaryotic cells (gram-negative and some gram-positive bacteria). It can be used to extract soluble proteins, membrane proteins, DNA, RNA, or metabolites.
ACROBiosystems GMP Salt Active GENIUS™Nuclease
GMP Salt Active GENIUS™Nuclease is expressed from Serratia marcescens E. coli.
ACROBiosystems Salt Active GENIUS™Nuclease, premium grade
Salt Active GENIUS™Nuclease, premium grade is expressed from Serratia marcescens E. coli.
Qiagen Beverly Inc E. COLI DNA LIGASE
DNA Ligase; E. Coli DNA Ligase; Conc. 10,000 U/ul; 2,500U; incl. 10X buffer
Creative Biomart Native Bacillus licheniformis Protease
AbbrProtease, Native (Bacillus licheniformis)AliasProteaseSourceBacillus licheniformisApplicationsThe product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination. It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies. This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.Product OverviewThe enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60°C.FormType VIII, lyophilized powder; Type I, aqueous solutionEnzyme Commission NumberEC 3.4.21.62